Titel
Rapid, high-yield enzymatic synthesis of n.c.a. 6-[18F]fluorodopamine (6-[18F]FDA) for in vivo application
Autor*in
Karsten Bamminger
Division of Nuclear Medicine, Department of Biomedical Imaging and Image-guided Therapy, Medical University of Vienna
Georgios Karanikas
Division of Nuclear Medicine, Department of Biomedical Imaging and Image-guided Therapy, Medical University of Vienna
... show all
Abstract
6-[18F]Fluorodopamine (6-[18F]FDA) proved valuable as a diagnostic tool for neuroendocrine and rare tumors, such as ganglioneuromas and pheochromocytomas, however, clinical application is still limited. So far, its radiosyntheses were impeded by the formation of side-products, low molar activity, multi-step reactions and the use of challenging precursors. Here, we describe a detailed enzymatic procedure for fast, simple and high yield synthesis of no carrier added 6-[18F]FDA from 6-[18F]FDOPA with high radiochemical yield of ~69 % and >95 % purity as determined by radio-HPLC and -TLC. The product formulation was stable and suitable for in vivo application.
Stichwort
Fluorine-186-FDOPA6-fluorodopamineRadiosynthesisEnzymaticTyrosine decarboxylase
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Nuclear Medicine and Biology
Band
114-115
ISSN
0969-8051
Erscheinungsdatum
2022
Seitenanfang
189
Seitenende
197
Publication
Elsevier BV
Erscheinungsdatum
2022
Zugänglichkeit
Rechte
Rechteangabe
© 2022 The Authors
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