Titel
Nutrient niche specificity for glycosaminoglycans is reflected in polysaccharide utilization locus architecture of gut Bacteroides species
Autor*in
Bela Hausmann
Joint Microbiome Facility, Medical University of Vienna
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Abstract
Introduction: Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides. GAG abundances are altered in some diseases such as inflammatory bowel diseases, which may affect microbial composition and activity, and it is therefore important to understand GAG utilization by members of the gut microbiota.
Methods: We used growth assays, transcriptomics, and comparative genomics to evaluate chondroitin sulfate (CS) and hyaluronan (HA) degradation ability by multiple gut Bacteroides species.
Results and discussion: We found that not all Bacteroides species able to degrade CS could also degrade HA, despite having lyases which act on both compounds. We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown. Furthermore, degradation of both compounds provides public goods for other Bacteroides, including non-degraders, suggesting that cooperative degradation as well as cross-feeding may be widespread in the mucosal glycan utilization clade.
Stichwort
hyaluronanchondroitin sulfateglycosaminoglycansbacteroidesintestine
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Frontiers in Microbiology
Band
13
ISSN
1664-302X
Erscheinungsdatum
2022
Publication
Frontiers Media SA
Projekt
Kod / Identifikator
P27831-B28
Projekt
Kod / Identifikator
741623
Fördergeber
Projekt
Kod / Identifikator
DOC 69
Erscheinungsdatum
2022
Zugänglichkeit
Rechte
Rechteangabe
© 2022 Overbeeke, Hausmann, Nikolov, Pereira, Herbold and Berry
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