Titel
Simple synthesis of massively parallel RNA microarrays via enzymatic conversion from DNA microarrays
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Abstract
RNA catalytic and binding interactions with proteins and small molecules are fundamental elements of cellular life processes as well as the basis for RNA therapeutics and molecular engineering. In the absence of quantitative predictive capacity for such bioaffinity interactions, high throughput experimental approaches are needed to sufficiently sample RNA sequence space. Here we report on a simple and highly accessible approach to convert commercially available customized DNA microarrays of any complexity and density to RNA microarrays via a T7 RNA polymerase-mediated extension of photocrosslinked methyl RNA primers and subsequent degradation of the DNA templates.
Stichwort
High-throughput screeningMicroarray analysisNucleic acidsRNA
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Nature Communications
Band
13
ISSN
2041-1723
Erscheinungsdatum
2022
Publication
Springer Science and Business Media LLC
Projekt
Kod / Identifikator
P23797
Projekt
Kod / Identifikator
P27275
Projekt
Kod / Identifikator
P30596
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Erscheinungsdatum
2022
Zugänglichkeit
Rechte
Rechteangabe
© The Author(s) 2022
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