Titel
Biochemometry identifies ostruthin as pluripotent antimicrobial and anthelmintic agent from masterwort
Autor*in
Cristina D. Cruz
Drug Research Program, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki
... show all
Abstract
The root extract of Peucedanum ostruthium (PO-E) was identified as a promising antibacterial source from a screening of 158 extracts against Staphylococcus aureus. It has also recently been shown to significantly decrease the survival of the nematode Caenorhabditis elegans. We used the biochemometric approach ELINA to investigate the phytochemical characteristics of the multicomponent mixture PO-E to identify the anti-infective constituent(s) targeting S. aureus and C. elegans. 1H NMR spectra of PO-E-derived microfractions were correlated with their respective bioactivity data. Heterocovariance analyses unambiguously identified ostruthin as an anti-staphylococcal constituent, which potently also inhibited Enterococcus spp.. ELINA demonstrated that anthelmintic activity was due to a combinatorial effect of ostruthin and isoimperatorin. A C. elegans-based survival and motility assay confirmed that isoimperatorin, imperatorin, and verapamil modulated the susceptibility of ostruthin. The combinatorial effect of these natural products was shown in larvae studies to be related to the function of the nematodes’ efflux pump.
Stichwort
Medical microbiologyDrugsBiochemistry
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
https://phaidra.univie.ac.at/o:2065347
Erschienen in
Titel
iScience
Band
26
Ausgabe
9
ISSN
2589-0042
Erscheinungsdatum
2023
Verlag
Elsevier BV
Projektnummer
P34028 – Austrian Science Fund (FWF)
Erscheinungsdatum
2023
Zugänglichkeit
Rechteangabe
© 2023 The Author(s)

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