Titel
Mdm38 protein depletion causes loss of mitochondrial K+/H+ exchange activity, osmotic swelling and mitophagy
Autor*in
Karin Nowikovsky
Autor*in
Siegfried Reipert
Autor*in
Rodney J Devenish
Monash University
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Abstract
Loss of the MDM38 gene product in yeast mitochondria results in a variety of phenotypic effects including reduced content of respiratory chain complexes, altered mitochondrial morphology and loss of mitochondrial K+/H+ exchange activity resulting in osmotic swelling. By use of doxycycline-regulated shut-off of MDM38 gene expression, we show here that loss of K+/H+ exchange activity and mitochondrial swelling are early events, associated with a reduction in membrane potential and fragmentation of the mitochondrial reticulum. Changes in the pattern of mitochondrially encoded proteins are likely to be secondary to the loss of K+/H+ exchange activity. The use of a novel fluorescent biosensor directed to the mitochondrial matrix revealed that the loss of K+/H+ exchange activity was immediately followed by morphological changes of mitochondria and vacuoles, the close association of these organelles and finally uptake of mitochondrial material by vacuoles. Nigericin, a K+/H+ ionophore, fully prevented these effects of Mdm38p depletion. We conclude that osmotic swelling of mitochondria triggers selective mitochondrial autophagy or mitophagy.
Stichwort
K+/H+ exchange, MDM38, mitophagy, osmotic swelling
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
https://phaidra.univie.ac.at/o:243628
Erschienen in
Titel
Cell Death and Differentiation
Erscheinungsdatum
01.01.2007
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