Titel
Changes in Oral Microbial Ecology of C57BL/6 Mice at Different Ages Associated with Sampling Methodology
Autor*in
Angélica Hernández-Arriaga
Institute of Animal Science, University of Hohenheim
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Abstract
The mouth is an important niche for bacterial colonization. Previous research used mouth microbiota to predict diseases like colon cancer and inflammatory bowel disease (IBD). It is still unclear how the sampling methodology influences microbial characterization. Our aim was to determine if the sampling methods, e.g., cotton swab or tissue biopsy, and the age influence the oral microbial composition of mice. Microbial DNA was extracted using a commercial kit and characterized targeting the 16s rRNA gene from mouth swabs and tissue biopsies from 2 and 15 months old C57BL/6 male mice kept in the same SPF facility. Our results show statistical different microbial community of the different ages, type of sampling, and the two fixed factors age x type of sample (p-value < 0.05). At the genus level, we identified that the genera Actinobacillus, Neisseria, Staphylococcus, and Streptococcus either increase or decrease in abundance depending on sampling and age. Additionally, the abundance of Streptococcus danieliae, Moraxella osloensis, and some unclassified Streptococcus was affected by the sampling method. While swab and tissue biopsies both identified the common colonizers of oral microbiota, cotton swabbing is a low-cost and practical method, validating the use of the swab as the preferred oral sampling approach.
Stichwort
oral microbiotaoral swaboral tissue biopsymicrobial ecologyagingsampling methodologies
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Microorganisms
Band
7
Ausgabe
9
ISSN
2076-2607
Erscheinungsdatum
2019
Publication
MDPI AG
Erscheinungsdatum
2019
Zugänglichkeit
Rechte
Rechteangabe
© 2019 by the authors
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