Conversion of Rutin, a Prevalent Dietary Flavonol, by the Human Gut Microbiota

Alessandra Riva; Ditta Kolimár; Andreas Spittler; Lukas Wisgrill; Craig W. Herbold; László Abrankó; David Berry

doi:10.3389/fmicb.2020.585428

Titel

Conversion of Rutin, a Prevalent Dietary Flavonol, by the Human Gut Microbiota

Autor*in

Alessandra Riva

Department für Mikrobiologie und Ökosystemforschung, Zentrum für Mikrobiologie und Umweltsystemwissenschaft, Universität Wien

Ditta Kolimár

Department of Applied Chemistry, Faculty of Food Science, Szent István University

Andreas Spittler

Research Lab, Core Facility Flow Cytometry, Department of Surgery, Medical University of Vienna

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Abstract

The gut microbiota plays a pivotal role in the conversion of dietary flavonoids, which can affect their bioavailability and bioactivity and thereby their health-promoting properties. The ability of flavonoids to metabolically-activate the microbiota has, however, not been systematically evaluated. In the present study, we used a fluorescence-based single-cell activity measure [biorthogonal non-canonical ammino acid-tagging (BONCAT)] combined with fluorescence activated cell sorting (FACS) to determine which microorganisms are metabolically-active after amendment of the flavonoid rutin. We performed anaerobic incubations of human fecal microbiota amended with rutin and in the presence of the cellular activity marker L-azidohomoalanine (AHA) to detect metabolically-active cells. We found that 7.3% of cells in the gut microbiota were active after a 6 h incubation and 26.9% after 24 h. We then sorted BONCAT-positive cells and observed an enrichment of Lachnospiraceae (Lachnoclostridium and Eisenbergiella), Enterobacteriaceae, Tannerellaceae, and Erysipelotrichaceae species in the rutin-responsive fraction of the microbiota. There was marked inter-individual variability in the appearance of rutin conversion products after incubation with rutin. Consistent with this, there was substantial variability in the abundance of rutin-responsive microbiota among different individuals. Specifically, we observed that Enterobacteriaceae were associated with conversion of rutin into quercetin-3-glucoside (Q-glc) and Lachnospiraceae were associated with quercetin (Q) production. This suggests that individual microbiotas differ in their ability to metabolize rutin and utilize different conversion pathways.

Stichwort

dietary bioactivesrutingut microbiotafluorescence activated cell sortingrutin metabolisminter-individual variability

Objekt-Typ

journal article

Sprache

Englisch [eng]

Persistent identifier

phaidra.univie.ac.at/o:1220811

DOI

10.3389/fmicb.2020.585428

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