Titel
A versatile depigmentation, clearing, and labeling method for exploring nervous system diversity
Autor*in
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Abstract
Tissue clearing combined with deep imaging has emerged as a powerful alternative to classical histological techniques. Whereas current techniques have been optimized for imaging selected nonpigmented organs such as the mammalian brain, natural pigmentation remains challenging for most other biological specimens of larger volume. We have developed a fast DEpigmEntation-Plus-Clearing method (DEEP-Clear) that is easily incorporated in existing workflows and combines whole system labeling with a spectrum of detection techniques, ranging from immunohistochemistry to RNA in situ hybridization, labeling of proliferative cells (EdU labeling) and visualization of transgenic markers. With light-sheet imaging of whole animals and detailed confocal studies on pigmented organs, we provide unprecedented insight into eyes, whole nervous systems, and subcellular structures in animal models ranging from worms and squids to axolotls and zebrafish. DEEP-Clear thus paves the way for the exploration of species-rich clades and developmental stages that are largely inaccessible by regular imaging approaches.
Stichwort
Multidisciplinary
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Science Advances
Band
6
Ausgabe
22
ISSN
2375-2548
Erscheinungsdatum
2020
Publication
American Association for the Advancement of Science (AAAS)
Projekt
Kod / Identifikator
P30035
Fördergeber
Projekt
Kod / Identifikator
P28970
Fördergeber
Projekt
Kod / Identifikator
P23102
Fördergeber
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Erscheinungsdatum
2020
Zugänglichkeit
Rechte
Rechteangabe
Copyright © 2020 The Authors, some rights reserved
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