Titel
LipidCreator workbench to probe the lipidomic landscape
Autor*in
Dominik Kopczynski
Leibniz-Institut für Analytische Wissenschaften – ISAS - e.V.
... show all
Abstract
Mass spectrometry (MS)-based targeted lipidomics enables the robust quantification of selected lipids under various biological conditions but comprehensive software tools to support such analyses are lacking. Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope labeling, optimize collision energy and generate in silico spectral libraries. We validate LipidCreator assays computationally and analytically and prove that it is capable to generate large targeted experiments to analyze blood and to dissect lipid-signaling pathways such as in human platelets.
Stichwort
LipidomicsMass spectrometrySoftware
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Nature Communications
Band
11
ISSN
2041-1723
Erscheinungsdatum
2020
Publication
Springer Science and Business Media LLC
Erscheinungsdatum
2020
Zugänglichkeit
Rechte
Rechteangabe
© The Author(s) 2020
Universität Wien | Universitätsring 1 | 1010 Wien | T
+43-1-4277-0