Titel
The Historical Development of Cultivation Techniques for Methanogens and Other Strict Anaerobes and Their Application in Modern Microbiology
Autor*in
Nikola Hanišáková
Laboratory of Anaerobic Microorganisms, Section of Microbiology, Department of Experimental Biology, Faculty of Science, Masaryk University
Monika Vítězová
Laboratory of Anaerobic Microorganisms, Section of Microbiology, Department of Experimental Biology, Faculty of Science, Masaryk University
Abstract
The cultivation and investigation of strictly anaerobic microorganisms belong to the fields of anaerobic microbial physiology, microbiology, and biotechnology. Anaerobic cultivation methods differ from classic microbiological techniques in several aspects. The requirement for special instruments, which are designed to prevent the contact of the specimen with air/molecular oxygen by different means of manipulation, makes this field more challenging for general research compared to working with aerobic microorganisms. Anaerobic microbiological methods are required for many purposes, such as for the isolation and characterization of new species and their physiological examination, as well as for anaerobic biotechnological applications or medical indications. This review presents the historical development of methods for the cultivation of strictly anaerobic microorganisms focusing on methanogenic archaea, anaerobic cultivation methods that are still widely used today, novel methods for anaerobic cultivation, and almost forgotten, but still relevant, techniques.
Stichwort
methaneanaerobesmethanogensbiogascultivation methods
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
phaidra.univie.ac.at/o:1623967
Erschienen in
Titel
Microorganisms
Band
10
Ausgabe
2
ISSN
2076-2607
Erscheinungsdatum
2022
Publication
MDPI AG
Erscheinungsdatum
2022
Zugänglichkeit
Rechte
Rechteangabe
© 2022 by the authors
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