Titel
Investigations on sub-structures within cavities of surface imprinted polymers using AFM and PF-QNM
Autor*in
... show all
Abstract
Investigations on lithographically formed cavities of surface-imprinted polymers (SIP) can help to gain deeper understanding on cell recognition with SIPs: it is known that surface topography and biomolecules transferred during surface imprinting contribute to cell adhesion. In this work, SIPs synthesized via two different imprinting techniques, namely stamp imprinting and polymerization of Pickering emulsions, were investigated and compared to each other, using atomic force microscopy (AFM) and Peak Force Quantitative Nano Mechanics (PF-QNM). We focused on SIPs based on poly(styrene-co-divinylbenzene) as model polymer and E. coli as model template for cell imprinting. Both imprinting approaches led to cavities that revealed nanostructures within the imprints. Stamp imprinting cavities feature low surface roughness and channel structures that resemble the negative pattern of the bacteria on the stamp and their filaments, while SIPs synthesized via polymerization of Pickering emulsions reveal globular nanostructures accumulating in the imprints. AFM phase imaging and adhesion mapping using PF-QNM show that these globular structures are remainders of the imprinted E. coli cells, most likely lipopolysaccarides, which is not observable in imprints resulting from stamp imprinting.
Stichwort
Condensed Matter PhysicsGeneral Chemistry
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
phaidra.univie.ac.at/o:1632170
Erschienen in
Titel
Soft Matter
Band
18
Ausgabe
11
ISSN
1744-683X
Erscheinungsdatum
2022
Seitenanfang
2245
Seitenende
2251
Publication
Royal Society of Chemistry (RSC)
Erscheinungsdatum
2022
Zugänglichkeit
Rechte
Universität Wien | Universitätsring 1 | 1010 Wien | T
+43-1-4277-0