Titel
Microbes from Mum: symbiont transmission in the tropical reef sponge Ianthella basta
Autor*in
J. Pamela Engelberts
Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, The University of Queensland, Australia
Muhammad A. Abdul Wahab
Australian Institute of Marine Science
Manuel Maldonado
Department of Marine Ecology, Centre for Advanced Studies of Blanes (CEAB-CSIC), Spain
... show all
Abstract
Most marine sponge species harbour distinct communities of microorganisms which contribute to various aspects of their host’s health and physiology. In addition to their key roles in nutrient transformations and chemical defence, these symbiotic microbes can shape sponge phenotype by mediating important developmental stages and influencing the environmental tolerance of the host. However, the characterisation of each microbial taxon throughout a sponge’s life cycle remains challenging, with several sponge species hosting up to 3000 distinct microbial species. Ianthella basta, an abundant broadcast spawning species in the Indo-Pacific, is an emerging model for sponge symbiosis research as it harbours only three dominant symbionts: a Thaumarchaeotum, a Gammaproteobacterium, and an Alphaproteobacterium. Here, we successfully spawned Ianthella basta, characterised its mode of reproduction, and used 16S rRNA gene amplicon sequencing, fluorescence in situ hybridisation, and transmission electron microscopy to characterise the microbial community throughout its life cycle. We confirmed I. basta as being gonochoric and showed that the three dominant symbionts, which together make up >90% of the microbiome according to 16S rRNA gene abundance, are vertically transmitted from mother to offspring by a unique method involving encapsulation in the peri-oocytic space, suggesting an obligate relationship between these microbes and their host.
Stichwort
Automotive Engineering
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
phaidra.univie.ac.at/o:1738446
Erschienen in
Titel
ISME Communications
Band
2
Ausgabe
1
ISSN
2730-6151
Erscheinungsdatum
2022
Publication
Springer Science and Business Media LLC
Erscheinungsdatum
2022
Zugänglichkeit
Rechte
Rechteangabe
© The Author(s) 2022
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