Titel
Missing Pieces in Structure Puzzles: How Hyperpolarized NMR Spectroscopy Can Complement Structural Biology and Biochemistry
Abstract
Structure determination lies at the heart of many biochemical research programs. However, the “giants”: X-ray diffraction, electron microscopy, molecular dynamics simulations, and nuclear magnetic resonance, among others, leave quite a few dark spots on the structural pictures drawn of proteins, nucleic acids, membranes, and other biomacromolecules. For example, structural models under physiological conditions or of short-lived intermediates often remain out of reach of the established experimental methods. This account frames the possibility of including hyperpolarized, that is, dramatically signal-enhanced NMR in existing workflows to fill these spots with detailed depictions. We highlight how integrating methods based on dissolution dynamic nuclear polarization can provide valuable complementary information about formerly inaccessible conformational spaces for many systems. A particular focus will be on hyperpolarized buffers to facilitate the NMR structure determination of challenging systems.
Stichwort
dissolution dynamic nuclear polarizationhyperpolarizationintegrative structural biologymethods developmentNMR spectroscopy
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
phaidra.univie.ac.at/o:1939173
Erschienen in
Titel
ChemBioChem
Band
24
Ausgabe
6
ISSN
1439-4227
Erscheinungsdatum
2023
Publication
Wiley
Fördergeber
Europäische Union (alle Programme) — 801936
Erscheinungsdatum
2023
Zugänglichkeit
Rechte
Rechteangabe
© 2023 The Authors
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