Titel
Gold‐FISH enables targeted NanoSIMS analysis of plant‐associated bacteria
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Abstract
Bacteria colonize plant roots and engage in reciprocal interactions with their hosts. However, the contribution of individual taxa or groups of bacteria to plant nutrition and fitness is not well characterized due to a lack of in situ evidence of bacterial activity.
To address this knowledge gap, we developed an analytical approach that combines the identification and localization of individual bacteria on root surfaces via gold-based in situ hybridization with correlative NanoSIMS imaging of incorporated stable isotopes, indicative of metabolic activity.
We incubated Kosakonia strain DS-1-associated, gnotobiotically grown rice plants with 15N–N2 gas to detect in situ N2 fixation activity. Bacterial cells along the rhizoplane showed heterogeneous patterns of 15N enrichment, ranging from the natural isotope abundance levels up to 12.07 at% 15N (average and median of 3.36 and 2.85 at% 15N, respectively, n = 697 cells).
The presented correlative optical and chemical imaging analysis is applicable to a broad range of studies investigating plant–microbe interactions. For example, it enables verification of the in situ metabolic activity of host-associated commercialized strains or plant growth-promoting bacteria, thereby disentangling their role in plant nutrition. Such data facilitate the design of plant–microbe combinations for improvement of crop management.
Stichwort
biological nitrogen fixationin situ hybridizationmicrobial activityNanoSIMSplant growth-promoting bacteriaplant–microbe interactionrhizosphere bacteria
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
phaidra.univie.ac.at/o:2037573
Erschienen in
Titel
New Phytologist
Band
240
Ausgabe
1
ISSN
0028-646X
Erscheinungsdatum
2023
Seitenanfang
439
Seitenende
451
Publication
Wiley
Fördergeber
Europäische Union (alle Programme) — 628361
Europäische Union (alle Programme) — 636928
Erscheinungsdatum
2023
Zugänglichkeit
Rechte
Rechteangabe
© 2023 The Authors
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