Titel
StressMatic: A Novel Automated System to Induce Depressive- and Anxiety-like Phenotype in Rats
Autor*in
Joana Martins-Macedo
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho
Autor*in
António Mateus-Pinheiro
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho
... show all
Abstract
Major depressive disorder (MDD) is a multidimensional psychiatric disorder that is estimated to affect around 350 million people worldwide. Generating valid and effective animal models of depression is critical and has been challenging for neuroscience researchers. For preclinical studies, models based on stress exposure, such as unpredictable chronic mild stress (uCMS), are amongst the most reliable and used, despite presenting concerns related to the standardization of protocols and time consumption for operators. To overcome these issues, we developed an automated system to expose rodents to a standard uCMS protocol. Here, we compared manual (uCMS) and automated (auCMS) stress-exposure protocols. The data shows that the impact of the uCMS exposure by both methods was similar in terms of behavioral (cognition, mood, and anxiety) and physiological (cell proliferation and endocrine variations) measurements. Given the advantages of time and standardization, this automated method represents a step forward in this field of preclinical research.
Stichwort
automated rackstress-exposureprotocols standardizationpreclinical research
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Cells
Band
12
Ausgabe
3
ISSN
2073-4409
Erscheinungsdatum
2023
Publication
MDPI AG
Projekt
Kod / Identifikator
101003187
Erscheinungsdatum
2023
Zugänglichkeit
Rechteangabe
© 2023 by the authors

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