RGS4 impacts carbohydrate and siderophore metabolism in Trichoderma reesei

Miriam Schalamun; Eva Maria Molin; Monika Schmoll

doi:10.1186/s12864-023-09467-2

Titel

RGS4 impacts carbohydrate and siderophore metabolism in Trichoderma reesei

Autor*in

Miriam Schalamun

AIT Austrian Institute of Technology GmbH, Bioresources Unit, Center for Health & Bioresources

Eva Maria Molin

AIT Austrian Institute of Technology GmbH, Bioresources Unit, Center for Health & Bioresources

Monika Schmoll

Zentrum für Mikrobiologie und Umweltsystemwissenschaft, Universität Wien

Abstract

Background: Adaptation to complex, rapidly changing environments is crucial for evolutionary success of fungi. The heterotrimeric G-protein pathway belongs to the most important signaling cascades applied for this task. In Trichoderma reesei, enzyme production, growth and secondary metabolism are among the physiological traits influenced by the G-protein pathway in a light dependent manner. Results: Here, we investigated the function of the SNX/H-type regulator of G-protein signaling (RGS) protein RGS4 of T. reesei. We show that RGS4 is involved in regulation of cellulase production, growth, asexual development and oxidative stress response in darkness as well as in osmotic stress response in the presence of sodium chloride, particularly in light. Transcriptome analysis revealed regulation of several ribosomal genes, six genes mutated in RutC30 as well as several genes encoding transcription factors and transporters. Importantly, RGS4 positively regulates the siderophore cluster responsible for fusarinine C biosynthesis in light. The respective deletion mutant shows altered growth on nutrient sources related to siderophore production such as ornithine or proline in a BIOLOG phenotype microarray assay. Additionally, growth on storage carbohydrates as well as several intermediates of the D-galactose and D-arabinose catabolic pathway is decreased, predominantly in light. Conclusions: We conclude that RGS4 mainly operates in light and targets plant cell wall degradation, siderophore production and storage compound metabolism in T. reesei.

Stichwort

Trichoderma reeseiHypocrea jecorinaRegulator of G-protein signalingCellulaseNutrient sensingLight responseStorage carbohydratesIron homeostasisSiderophore

Objekt-Typ

journal article

Sprache

Englisch [eng]

Persistent identifier

phaidra.univie.ac.at/o:2064256

DOI

10.1186/s12864-023-09467-2

Erschienen in

Titel

BMC Genomics

Band