Titel
The phageome in normal and inflamed human skin
Autor*in
M. Wielscher
Department of Dermatology, Medical University of Vienna
Autor*in
K. Pfisterer
Department of Dermatology, Medical University of Vienna
Autor*in
D. Samardzic
Department of Dermatology, Medical University of Vienna
... show all
Abstract
Dysbiosis of skin microbiota drives the progression of atopic dermatitis (AD). The contribution of bacteriophages to bacterial community compositions in normal and inflamed skin is unknown. Using shotgun metagenomics from skin swabs of healthy individuals and patients with AD, we found 13,586 potential viral contiguous DNA sequences, which could be combined into 164 putative viral genomes including 133 putative phages. The Shannon diversity index for the viral metagenome-assembled genomes (vMAGs) did not correlate with AD. In total, we identified 28 vMAGs that differed significantly between normal and AD skin. Quantitative polymerase chain reaction validation of three complete vMAGs revealed their independence from host bacterium abundance. Our data indicate that normal and inflamed skin harbor distinct phageomes and suggest a causative relationship between changing viral and bacterial communities as a driver of skin pathology.
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
phaidra.univie.ac.at/o:2068501
Erschienen in
Titel
Science Advances
Band
9
Ausgabe
39
ISSN
2375-2548
Erscheinungsdatum
2023
Publication
American Association for the Advancement of Science (AAAS)
Erscheinungsdatum
2023
Zugänglichkeit
Rechteangabe
© 2023 The Authors, some rights reserved

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