Titel
Identification of inulin-responsive bacteria in the gut microbiota via multi-modal activity-based sorting
Autor*in
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Abstract
Prebiotics are defined as non-digestible dietary components that promote the growth of beneficial gut microorganisms. In many cases, however, this capability is not systematically evaluated. Here, we develop a methodology for determining prebiotic-responsive bacteria using the popular dietary supplement inulin. We first identify microbes with a capacity to bind inulin using mesoporous silica nanoparticles functionalized with inulin. 16S rRNA gene amplicon sequencing of sorted cells revealed that the ability to bind inulin was widespread in the microbiota. We further evaluate which taxa are metabolically stimulated by inulin and find that diverse taxa from the phyla Firmicutes and Actinobacteria respond to inulin, and several isolates of these taxa can degrade inulin. Incubation with another prebiotic, xylooligosaccharides (XOS), in contrast, shows a more robust bifidogenic effect. Interestingly, the Coriobacteriia Eggerthella lenta and Gordonibacter urolithinfaciens are indirectly stimulated by the inulin degradation process, expanding our knowledge of inulin-responsive bacteria.
Stichwort
Microbial ecologyMicrobiome
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
phaidra.univie.ac.at/o:2070231
Erschienen in
Titel
Nature Communications
Band
14
ISSN
2041-1723
Erscheinungsdatum
2023
Publication
Springer Science and Business Media LLC
Fördergeber
Europäische Union (alle Programme) — 741623
Erscheinungsdatum
2023
Zugänglichkeit
Rechte
Rechteangabe
© The Author(s) 2023
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