Titel
Scaling up a targeted exposome LC-MS/MS biomonitoring method by incorporating veterinary drugs and pesticides
Autor*in
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Abstract
Humans are exposed to a cocktail of food-related and environmental contaminants, potentially contributing to the etiology of chronic diseases. Better characterizing the “exposome” is a challenging task and requires broad human biomonitoring (HBM). Veterinary drugs (VDs)/antibiotics, widely used and regulated in food and animal production, however, are typically not yet included in exposomics workflows. Therefore, in this work, a previously established multianalyte liquid chromatography-tandem mass spectrometry (LC-MS/MS) method covering >80 diverse xenobiotics was expanded by >40 VDs/antibiotics and pesticides. It was investigated if the generic workflow allowed for the successful integration of a high number of new analytes in a proof-of-principle study. The expanded method was successfully in-house validated and specificity, matrix effects, linearity, intra- and inter-day precision, accuracy, limits of quantification, and detection were evaluated. The optimized method demonstrated satisfactory recovery (81–120%) for most of the added analytes with acceptable RSDs (<20%) at three spiking levels. The majority of VDs/antibiotics and pesticides (69%) showed matrix effects within a range of 50–140%. Moreover, sensitivity was excellent with median LODs and LOQs of 0.10 ng/mL and 0.31 ng/mL, respectively. In total, the expanded method can be used to detect and quantify more than 120 highly diverse analytes in a single analytical run. To the best of the authors’ knowledge, this work represents the first targeted biomonitoring method integrating VDs with various other classes of pollutants including plasticizers, PFAS, bisphenols, mycotoxins, and personal care products. It demonstrates the potential to expand targeted multianalyte methods towards additional groups of potentially toxic chemicals.
Stichwort
Mass spectrometryBiomarkers of exposureExposome researchVeterinary drugs/antibioticsFood safetyXenobiotics
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
phaidra.univie.ac.at/o:2080654
Erschienen in
Titel
Analytical and Bioanalytical Chemistry
Band
416
Ausgabe
19
ISSN
1618-2642
Erscheinungsdatum
2024
Seitenanfang
4369
Seitenende
4382
Publication
Springer Science and Business Media LLC
Fördergeber
Erscheinungsdatum
2024
Zugänglichkeit
Rechte
Rechteangabe
© The Author(s) 2024
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