Titel
Molecular profiles, sources and lineage restrictions of stem cells in an annelid regeneration model
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Abstract
Regeneration of missing body parts can be observed in diverse animal phyla, but it remains unclear to which extent these capacities rely on shared or divergent principles. Research into this question requires detailed knowledge about the involved molecular and cellular principles in suitable reference models. By combining single-cell RNA sequencing and mosaic transgenesis in the marine annelid Platynereis dumerilii, we map cellular profiles and lineage restrictions during posterior regeneration. Our data reveal cell-type specific injury responses, re-expression of positional identity factors, and the re-emergence of stem cell signatures in multiple cell populations. Epidermis and mesodermal coelomic tissue produce distinct putative posterior stem cells (PSCs) in the emerging blastema. A novel mosaic transgenesis strategy reveals both developmental compartments and lineage restrictions during regenerative growth. Our work supports the notion that posterior regeneration involves dedifferentiation, and reveals molecular and mechanistic parallels between annelid and vertebrate regeneration.
Stichwort
Adult stem cellsMultipotent stem cellsRegeneration
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Nature Communications
Band
15
ISSN
2041-1723
Erscheinungsdatum
2024
Publication
Springer Science and Business Media LLC
Projekt
Kod / Identifikator
I2972
Projekt
Kod / Identifikator
F78
Projekt
Kod / Identifikator
DOC 72
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Erscheinungsdatum
2024
Zugänglichkeit
Rechte
Rechteangabe
© The Author(s) 2024
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