Titel
Differentiation between wild boar and domestic pig in food by targeting two gene loci by real-time PCR
Autor*in
Walter Mayer
Department of Molecular Biology and Microbiology, Institute for Food Safety Vienna, Austrian Agency for Health and Food Safety
Kirsten Kerkhoff
Impetus GmbH & Co. Bioscience KG
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Abstract
Studies indicate that many meat products are not authentic, most frequently because the meat species differ from those given on the food labels. At present, DNA based methods play the most important role in meat species authentication. Discrimination of wild boar and domestic pig meat in food is challenging because it is differentiation on the subspecies level. We developed and validated two singleplex real-time PCR assays targeting SNP rs81416363 on chromosome 9 and a duplex real-time PCR assay targeting SNP g.299084751 C > T in the NR6A1 gene located on chromosome 1. The singleplex real-time PCR assays led to some ambiguous results for Mangalica and Krškopolje pig breeds and wild boar individuals from Germany, the duplex real-time PCR assay particularly for the Turopolje pig breed. We demonstrate that the probability of misclassification can be substantially reduced if the results of both the singleplex real-time PCR assays and the duplex real-time PCR assay are taken into consideration. 86 (91.5%) of a total of 94 individuals, comprising 64 domestic pigs (14 different breeds and 6 cross-breeds) and 30 wild boars (from Austria, Germany, Romania, USA and Estonia), were classified correctly.
Stichwort
GeneticsMolecular biology
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Scientific Reports
Band
9
ISSN
2045-2322
Erscheinungsdatum
2019
Publication
Springer Science and Business Media LLC
Erscheinungsdatum
2019
Zugänglichkeit
Rechte
Rechteangabe
© The Author(s) 2019
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