Titel
3D Dissection of Structural Membrane-Wall Contacts in Filamentous Moss Protonemata
Autor*in
Abstract
In conventional light microscopy, the adjacent cell walls of filamentous moss protonemata are seen from its narrow side thereby obscuring the major area of cell–cell connection. Optical sectioning, segmentation and 3D reconstructions allow the tilting and rotation of intracellular structures thereby greatly improving our understanding of interaction between organelles, membranes and the cell wall. Often, the findings also allow for conclusions on the respective functions. The moss Physcomitrium (Physcomitrella) patens is a model organism for growth, development and morphogenesis. Its filamentous protonemata are ideal objects for microscopy. Here, we investigated the cell wall between two neighboring cells and the connection of membranes towards this wall after plasmolysis in 0.8 M mannitol. An m-green fluorescent protein (GFP)-HDEL cell line was used to visualize the endoplasmatic reticulum (ER), the plasma membrane (PM) was stained with FM4-64. Our studies clearly show the importance of cell–cell contacts in P. patens protonemata. In 86% of the investigated cell pairs, at least one of the protoplasts remained fully attached to the adjacent cell wall. By tilting of z-stacks, volume renderings and 3D reconstructions, we visualized the amount of attached/detached PM and ER components after plasmolysis and membrane piercings through the wall of cell neighbors.
Stichwort
mossmembrane-wall contactPhyscomitrella sp.osmotic stresscaulonemachloronema3D imaging
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
International Journal of Molecular Sciences
Band
22
Ausgabe
1
ISSN
1422-0067
Erscheinungsdatum
2020
Seitenanfang
158
Publication
MDPI AG
Erscheinungsdatum
2020
Zugänglichkeit
Rechte
Rechteangabe
© 2020 by the authors
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