International Conference on Quantum Physics of Nature (QuPoN 2015) Mitschnitt einer Veranstaltung der Fakultät für Physik am Dienstag, dem 19. Mai 2015 im Auditorium Maximum der Universität Wien Teil 21. Boltzmann Lecture 2015 mit Stefan W. Hell: "Optical microscopy: the resolution revolution" Mit einleitenden Worten von Robin Golser (Dekan der Fakultät für Physik), Heinz Engl (Rektor der Universität Wien) und Markus Arndt (Fakultät für Physik) Schnitt: Daniel Winkler Abstract: Throughout the 20th century it was widely accepted that a light microscope relying on conventional optical lenses cannot discern details that are much finer than about half the wavelength of light (200-400 nm), due to diffraction. However, in the 1990s, the viability to overcome the diffraction barrier was realized and microscopy concepts defined that can resolve fluorescent features down to molecular dimensions. In this lecture, I will discuss the simple yet powerful principles that allow neutralizing the limiting role of diffraction. In a nutshell, feature molecules residing closer than the diffraction barrier are transferred to different (quantum) states, usually a bright fluorescent state and a dark state, so that they become discernible for a brief period of detection. Thus, the resolution-limiting role of diffraction is overcome, and the interior of transparent samples, such as living cells and tissues, can be imaged at the nanoscale. Stefan W. Hell ist Direktor am Max-Planck-Institut für Biophysikalische Chemie in Göttingen und Nobelpreisträger für Chemie des Jahres 2014. INHALT ====== Kapitel Titel Position --------------------------------------------------------------------- 1. Vorspann 00:00:00 2. R. Golser: Eröffnung 00:00:16 3. H. Engl: Begrüßung 00:02:27 4. M. Arndt: Einleitende Worte 00:04:27 5. Limits of light microscopy 00:11:48 6. Overcoming Abbe's barrier 00:26:45 7. Examples 00:37:33 8. Spatial resolution of STED microscopes 00:42:26 9. What does it take to get the best resolution? 00:56:09

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